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cap1 analog  (New England Biolabs)


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    Structured Review

    New England Biolabs cap1 analog
    Cap1 Analog, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 275 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/analog/m7G(5)ppp(5)G+RNA+Cap+Structure+Analog/pmc13605572-126-8-10
    Average 95 stars, based on 275 article reviews
    cap1 analog - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Virus:

    Article Title: Atomic-resolution structure of a chimeric Powassan tick-borne flavivirus
    Article Snippet: .. The cDNA clone was linearized with Xho I enzyme (NEB, #N0146), and RNA transcripts of the yPOWV virus were generated by in vitro transcription using SP6 RNA polymerase (NEB, #M0207) with Cap A analog (NEB, #S1406). .. The in vitro transcribed RNA was introduced into BHK-15 cells by electroporation using Bio-Rad Gene Pulser Xcell using a 4-mm cuvette (Bio-Rad, #1652081).

    Article Title: Atomic-resolution structure of a chimeric Powassan tick-borne flavivirus.
    Article Snippet: .. The cDNA clone was linearized with Xho I enzyme (NEB, #N0146), and RNA transcripts of the yPOWV virus were generated by in vitro transcription using SP6 RNA polymerase (NEB, #M0207) with Cap A analog (NEB, #S1406). .. The in vitro transcribed RNA was introduced into BHK- 15 cells by electroporation using BioRad Gene Pulser Xcell using a 4- mm cuvette (Bio- Rad, #1652081).

    Generated:

    Article Title: Atomic-resolution structure of a chimeric Powassan tick-borne flavivirus
    Article Snippet: .. The cDNA clone was linearized with Xho I enzyme (NEB, #N0146), and RNA transcripts of the yPOWV virus were generated by in vitro transcription using SP6 RNA polymerase (NEB, #M0207) with Cap A analog (NEB, #S1406). .. The in vitro transcribed RNA was introduced into BHK-15 cells by electroporation using Bio-Rad Gene Pulser Xcell using a 4-mm cuvette (Bio-Rad, #1652081).

    Article Title: Atomic-resolution structure of a chimeric Powassan tick-borne flavivirus.
    Article Snippet: .. The cDNA clone was linearized with Xho I enzyme (NEB, #N0146), and RNA transcripts of the yPOWV virus were generated by in vitro transcription using SP6 RNA polymerase (NEB, #M0207) with Cap A analog (NEB, #S1406). .. The in vitro transcribed RNA was introduced into BHK- 15 cells by electroporation using BioRad Gene Pulser Xcell using a 4- mm cuvette (Bio- Rad, #1652081).

    In Vitro:

    Article Title: Atomic-resolution structure of a chimeric Powassan tick-borne flavivirus
    Article Snippet: .. The cDNA clone was linearized with Xho I enzyme (NEB, #N0146), and RNA transcripts of the yPOWV virus were generated by in vitro transcription using SP6 RNA polymerase (NEB, #M0207) with Cap A analog (NEB, #S1406). .. The in vitro transcribed RNA was introduced into BHK-15 cells by electroporation using Bio-Rad Gene Pulser Xcell using a 4-mm cuvette (Bio-Rad, #1652081).

    Article Title: Generation of a Lineage II Powassan Virus (Deer Tick Virus) cDNA Clone: Assessment of Flaviviral Genetic Determinants of Tick and Mosquito Vector Competence
    Article Snippet: .. For NY99 WNV, DTV, and DTV-prME/WNV infectious clones, the linearized product was then utilized as a template for in vitro transcription of positive-sense viral RNA from a T7 RNA polymerase promoter with an analog A cap m 7 G(5′)ppp(5′)A (New England Biolabs) as described for WNV cDNA. ..

    Article Title: Atomic-resolution structure of a chimeric Powassan tick-borne flavivirus.
    Article Snippet: .. The cDNA clone was linearized with Xho I enzyme (NEB, #N0146), and RNA transcripts of the yPOWV virus were generated by in vitro transcription using SP6 RNA polymerase (NEB, #M0207) with Cap A analog (NEB, #S1406). .. The in vitro transcribed RNA was introduced into BHK- 15 cells by electroporation using BioRad Gene Pulser Xcell using a 4- mm cuvette (Bio- Rad, #1652081).

    Clone Assay:

    Article Title: Generation of a Lineage II Powassan Virus (Deer Tick Virus) cDNA Clone: Assessment of Flaviviral Genetic Determinants of Tick and Mosquito Vector Competence
    Article Snippet: .. For NY99 WNV, DTV, and DTV-prME/WNV infectious clones, the linearized product was then utilized as a template for in vitro transcription of positive-sense viral RNA from a T7 RNA polymerase promoter with an analog A cap m 7 G(5′)ppp(5′)A (New England Biolabs) as described for WNV cDNA. ..



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